Ime- and dose-dependent manner (Figure two).Figure 2. The effects of exposure to simazine for 12, 24, and 48 h on tyrosine hydroxylase (DYT5b) (a); Figure two. The effects of exposure to simazine for 12, 24, and 48 h on tyrosine hydroxylase (DYT5b) aromatic amino acid decarboxylase (AADC) (b); dopamine transporter (DAT) (c) and monoamine (a); aromatic amino acid decarboxylase (AADC) (b); dopamine transporter (DAT) (c) and monoamine vesicular transporter 2 (VMAT2) (d); monoamine oxidase (MAO) (e); catechol-O-methyl transferase vesicular transporter two (VMAT2) (d); monoamine oxidase (MAO) (e); catechol-O-methyl transferase (COMT) (f) mRNA relative levels fold change to manage in MN9D cells had been presented. Bars indicate (COMT) (f) mRNA relative levels fold modify to control in MN9D cells have been presented. Bars indicate imply S.E.M. statistically considerable difference compared with all the control, p 0.05, 3 repeated mean S.E.M. statistically significant distinction compared using the manage, p 0.05, 3 repeated experiments for each group, n = three. experiments for each group, n = three.Figure two shows the impact of simazine exposure for 12 and 24 h on DYT5b (a), AADC (b), DAT (c) and Figure two shows the effectCOMT (f) mRNA levels. 12 and 24 h on DYT5b (a), AADCsignificantly VMAT2 (d), MAO (e), of simazine exposure for DYT5b (a) mRNA levels was (b), DAT (c) and VMAT2 (d), MAO (e), COMT (f) mRNA levels. for 12 h mRNA levels was control (p 0.05); all enhanced following exposure to 600 simazineDYT5b (a)compared with thesignificantly enhanced following exposure to 600 levels for 24 h were considerably elevated compared together with the control dose groups of DYT5b mRNAsimazine for 12 h compared with the manage (p 0.05); all dose groups of DYT5b mRNA levels mRNA level were considerably improved following exposure(p 0.05); AADC (b) (p 0.05); AADC (b) for 24 h were considerably improved compared together with the manage to 300 and 600 mRNA level 12 and 24 h compared with the controls (p 0.05). DAT (c) and VMAT2 (d) mRNA levels simazine for had been significantly increased following exposure to 300 and 600 simazine for 12 and 24 h compared with all the controls (p 0.IRE1 Protein Accession 05).EGF Protein Accession DAT (c) and VMAT2 (d) mRNA levels had been drastically enhanced have been significantly improved following exposure to 300 and 600 simazine for 12 h compared with following exposure to DAT and VMAT2 mRNA levels compared using the controls (p following the controls (p 0.PMID:32180353 05);300 and 600 simazine for 12 h were also considerably increased0.05); DAT and VMAT2 mRNA and were also drastically improved following exposure to (p 0.05) (c and d). exposure to 150, 300levels600 simazine for 24 h compared together with the controls 150, 300 and 600 simazine for 24 h compared levels controls (p 0.05) (c 300 and 600 simazine for 12 in cells MAO and COMT mRNA with thein cells exposed to and d). MAO and COMT mRNA levelsh had been exposed to 300 and 600 compared with the controls (p 0.05) and exposed to the same dose of drastically decreased simazine for 12 h have been drastically decreased compared with the controls (p 0.05) and 24 h had been also very same dose of simazine for 24 h werewith considerably decreased (d and e). simazine for exposed for the drastically decreased compared also the controls (p 0.05) compared with all the controls (p 0.05) (d of these Having said that, the significantly decreasedgenes have been exposure to Having said that, the mRNA levels and e). genes have been mRNA levels of these following significantly decreased followi.