Study, and also the maximum concentrations of UA released in line with the MICs observed for the UA-loaded dendrimer NPs and determined by its DL and release profile [47]. The final two columns include the values of selectivity indices (SI) of UA and YTX-465 Autophagy UA-G4K NPs, respectively. UA MW 456.7 /mL, 2, four.four two, four.4 2, 4.four 4, 8.8 two, four.4 two, 4.4 four, 8.8 four, 8.8 UA-G4K MW 30,069 /mL, 64, two.1 128, four.3 128, four.3 64, 2.1 32, 1.1 32, 1.1 128, four.three 64, 2.1 Max UA Released two /mL, 17.7, 38.eight 35.four, 77.6 35.4, 77.six 17.7, 38.8 eight.9, 19.4 eight.9, 19.4 17.7, 38.eight 17.7, 38.eight Reference Antibiotic /mL, 128 (366.three) four 512 (353.three) 5 0.5 (1.41.four) four two.0 (1.4) five 0.5 (1.41.four) 4 two.0 (1.four) 5 128 (366.3) 4 512 (353.three) five 0.five (1.41.four) four 2.0 (1.four) five 0.five (1.41.4) 4 two.0 (1.four) five 0.5 (1.41.four) four 2.0 (1.4) five 0.5 (1.41.four) four two.0 (1.four) five 12.five 12.five 12.5 6.two 12.5 12.5 six.2 six.2 46.1 22.five 22.five 46.1 88.1 88.1 22.five 46.1 SI three UA SI three UA-G4KMIC ValuesE. faecalis 1 E. faecalis four # E. faecalis eight # E. faecalis 79 E. faecalis 108 # E. faecalis 200 # E. faecalis 261 # E. faecalis 425 #Pharmaceutics 2021, 13,8 ofTable three. Cont. UA MW 456.7 /mL, 2, 4.4 2, four.four 2, 4.4 two, 4.4 2, four.4 4, eight.8 2, four.four two, four.four two, four.4 two, four.four four, eight.eight 4, eight.8 2, 4.four 2, 4.four 2, 4.four two, four.4 four, 8.eight two, four.four four, eight.8 UA-G4K MW 30,069 /mL, 128, four.three 64, two.1 64, 2.1 32, 1.1 32, 1.1 32, 1.1 64, 2.1 32, 1.1 32, 1.1 16, 0.five 16, 0.five 32, 1.1 32, 1.1 16, 0.5 16, 0.five 16, 0.5 32, 1.1 64, two.1 64, two.1 Max UA Released 2 /mL, 35.4, 77.6 17.7, 38.eight 17.7, 38.eight eight.9, 19.4 eight.9, 19.four 8.9, 19.four 17.7, 38.eight 8.9, 19.4 8.9, 19.4 4.5, 9.7 4.five, 9.7 8.9, 19.four eight.9, 19.four 4.5, 9.7 4.5, 9.7 four.five, 9.7 eight.9, 19.4 17.7, 38.8 17.7, 38.8 Reference Antibiotic /mL, 128 (366.three) 4 512 (353.3) 5 128 (366.three) 4 512 (353.3) 5 0.five (1.41.4) four two.0 (1.4) five 128 (366.3) four 512 (353.3) five 128 (366.3) 4 512 (353.3) 5 128 (366.3) four 512 (353.3) five 128 (366.three) four 512 (353.three) 5 128 (366.3) four 512 (353.three) five N.R. N.R. N.R. N.R. N.R. N.R. N.R. N.R. N.R. N.R. N.R. 12.5 12.5 12.five 12.five 12.5 six.two 12.five 12.five 12.5 12.five six.two six.2 12.5 12.5 12.five 12.5 6.2 12.five six.2 22.five 46.1 46.1 88.1 88.1 88.1 46.1 88.1 88.1 193.8 193.8 88.1 88.1 193.eight 193.8 193.8 88.1 46.1 46.1 SI three UA SI 3 UA-G4KMIC ValuesE. faecalis 19 , E. faecalis 51 , E. faecium 21 # E. faecium 43 E. faecium 152 E. faecium 185 E. faecium 341 E. faecium 3 , E. gallinarum 150 E. gallinarum 294 E. gallinarum 295 E. casseliflavus 184 E. casseliflavus 296 E. casseliflavus 159 E. avium 119 E. avium 122 E. avium 167 E. durans 103 E. duransExperiments have been performed in triplicate, the concordance degree was 3/3, and D was zero; two derived from the MICs observed for UA-G4K soon after 24 h and according to the DL and UA released from UA-G4K NPs [47]; 3 SI = selectivity index (LD50 /MIC) exactly where LD50 was calculated working with data from cytotoxicity experiments on human keratinocytes soon after 24 h exposure; # indicates vancomycin susceptibility (VSE); indicates vancomycin resistance (VRE); indicates strains of YC-001 medchemexpress marine origin isolated from seawater of your Ligurian west coast; four ampicillin; 5 vancomycin; N.R. = not reported.As could be observed in Table 3, UA-G4K NPs had been really potent against all clinical and marine isolates with the Enterococcus genus tested. In reality, except for E. faecalis four (MIC = four.three ), the UA-G4K NPs displayed pretty low MICs (0.five.1 ), which were 2.15.6-fold reduced than these displayed by the untreated UA. This fascinating result is on account of the high DL of UA-G4K (which resulted inside the presence of 33 UA moles per mole of dendrimer) and to the favorable UA release profil.