cases reported in this paper. 2) The lack of association between tobacco and cancer diagnosis could be 1799948-06-3 cost surprising. However, given the previous diagnosis of CAD, most patients had given up smoking, thus reducing the possibility of finding such a relationship in this population. 3) Finally, plasma withdrawn during the acute index event has not been studied, because at this point the population was composed of patients with ST-elevation myocardial infarction and Non-ST-elevation Acute Coronary Syndrome. These are clinical entities with differences in the pathophysiology and management. Given this heterogeneity, mixing plasma findings at this stage could have introduced a bias in the results.
Soybean (Glycine max (L.) Merr.) is an important source of plant protein and oil. However, low yield is a key factor restricting its development. The utilization of soybean heterosis is probably one of the potential approaches in the future yield breakthrough as was the situation in rice breeding in China. Cytoplasmic male sterility (CMS) plays an important role in the production of hybrid seeds [1]. However, the molecular mechanism of CMS in soybean remains unclear. The transcriptome is the complete set of transcripts in a cell at a specific developmental stage or physiological condition, which can provide information on gene expression and gene regulation [2]. Transcriptome sequencing (RNA-seq) is a recently developed high-performance and comprehensive method of transcriptome analysis [3, 4]. Transcriptome analysis using RNA-seq technology has allowed for the comparison and analysis of thousands of genes within one experiment [5]. Liu et al. [6] analyzed differentially expressed genes between chili pepper cytoplasmic male sterile line 121A and its near-isogenic line-restorer line 121C at the transcriptional level using Solexa/Illumina technology, and found a group of key genes and significant pathways associated with male sterility. Wei et al. [7] conducted transcriptome analysis of differentially expressed genes in the process of development in wild type and nuclear male sterile cotton anthers using digital gene expression profiles, and illustrated that many key genes involved in anther development showed the opposite gene expression patterns in GMS mutant anthers compared with that of wild type anthers at the same development stage. Yan et al. [8] conducted analysis of genome-wide and high-throughput transcriptome 
sequencing on young floral buds of B. napus CMS line Nsa and its novel restorer line NR1 using Solexa/Illumina techniques, and found a group of candidate genes associated with male sterility. An et al. [9] compared the genomic expression profiles of fertile and sterile young flower buds of pol-CMS in B.napus by RNA-Seq,and found some unigenes controlling anther development were dramatically downregulated in sterile buds. However, there is no related report on CMS in soybean so far. The soybean cytoplasmic male sterile line NJCMS1A was developed through consecutive backcross procedures with the cultivar N8855 as donor parent and N2899 (designated as NJCMS1B afterwards) as recurrent parent [102]. So NJCMS1A and NJCMS1B were a pair of near-isogenic lines and fit for the study on the molecular mechanism of CMS in soybean. In the present paper, we tried to find important differentially expressed genes and metabolism pathways might related to the soybean CMS through the comparative transcriptome analysis between the flower buds of NJCMS1A and t