three.6.06 6.40 7.80 7.0.52 0.21 2.24 nd11.10 48.80 3.30 127.Soybean cropNatural pastureEntre R s i (Paran a Crdoba o (Corral de Bustos) Crdoba o (Corral de Bustos) Crdoba o (Corral de Bustos) Salta (Embarcacin) o Salta (Joaqu V. i Gonz ez) a Santiago del Estero (Quimil iSoybean cropTable 1: Continued. rep-PCR group ARDRA cluster Species assignments Partial 16S rDNA sequence (accession number) OM ( ) Soil chemical parameters pH EC (mS cm-1 ) P (ppm)Geographical originSampling siteIsolateSoybean crop 2 HQ541448 HQ623179 four four five five 5 6 IV IV IV HQ623178 IV IV IV A. salinestris A. salinestris A. salinestris A. salinestris A. salinestris A. salinestrisAT2 AT16 AT17 AT3 three 3nd nd nd IV A. salinestris A. salinestris A. salinestris A. salinestris2.78 1.09 two.81 0.19 2.ten 1.00 0.7.16 7.50 7.70 eight.77 7.43 eight.28 eight.nd 0.48 1.50 0.28 0.54 0.94 0.104.20 7.40 43.50 4.80 7.00 three.00 4.Organic pastureATRiver bankATSide of routeaATSoybean cropATSoybean cropATChubut (Puerto Madryn) Chubut (Villa Ameghino) Jujuy (Tilcara) Santa Fe (Videla) Santa Fe (Videla) Jujuy (Tilcara)Side of routeaATaBuenos Aires, Crdoba, Entre R s, and Santa Fe are provinces of the Pampas region; Jujuy, Salta, and Santiago del Estero are provinces in the Northwest region, Chubut is a province from Patagonia area of o i Argentina. Corresponded for the exact same soil sample. OM: organic matter; EC: electrical conductivity; P: extractable phosphorus; nd: not determined.The Scientific World JournalThe Scientific Planet JournalSimilarity ( ) 60 80 one hundred AT4 AT9 AT25 AT27 AT28 AT30 AT43 I II ATBNM 272 A.chroococcummAT33 NRRL B-14627A.vinelandii III NRRL B-14641A.vinelandii NRRL B-14644 A.vinelandii AT12 AT38 AT37 AT10 AT14 AT29 IV AT19 AT42 ATFigure 2: Amplified ribosomal DNA restriction evaluation (ARDRA) of Azotobacter representative strains of each and every rep-PCR group and reference strains. The dendrogram depending on evaluation of restriction patterns of 16S rDNA obtained with HhaI was constructed using the GelCompar II program and also the Dice ( ) pairwise coefficient of similarity plus the UPGMA algorithm. Clusters have been defined in the 80 similarity level. The cophenetic correlation value for this dendrogram was A. armeniacus DSM 2284T (99 identity), and also the 4 strains in cluster IV (AT18, AT19, AT37, and AT42) have been associated to A.4-Thiouridine Protocol salinestris ATCC 49674T (99-100 identity).Lauroylsarcosine Protocol Summarizing, according to the outcomes obtained by repPCR, ARDRA, and partial sequencing with the 16S ribosomal gene, the 15 isolates of group 1 of rep-PCR (Figure 1) have been classified as A.PMID:23795974 chroococcum, the 3 isolates of group 2 as A. armeniacus, and also the 13 isolates included in groups three to 6 as A. salinestris. three.four. Siderophore and Phytohormone Production, Phosphate Solubilization, and Nitrogenase Activity. All the 18 strains tested exhibited a color transform from blue to orange in CAS medium, that is indicative of siderophore production. Phosphate-solubilizing activity was not evident in any with the Azotobacter strains assayed, independently in the medium utilized (data not shown). All preselected strains were assayed for auxin production in LGSP medium applying the Salkowski reagent technique. Following 1 day of growth (108 cfu mL-1 ), all bacterial strains made low levels of auxin (0.96 g mL-1 to 2.64 g mL-1 ) (Table two). An important increase wasobserved after two and three days of development, without having any adjustments in cfu mL-1 (information not shown). Ultimately, bacterial strains differed within the levels of auxin excreted to the culture medium at the end of the ass.