Ommercial sources [Table 1]. PTE, CTE, XTL, PME, GCE, TDE (98 , purity) were
Ommercial sources [Table 1]. PTE, CTE, XTL, PME, GCE, TDE (98 , purity) had been acquired from Shanghai Tauto Biotech Co., Ltd. ATP was obtained from Shenzhen Meihe Biotech Co., Ltd. CDE and DCE (98 , purity) were bought from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). THP, IMP and ISO were obtained from National Institute to the Management of Pharmaceutical and Biological BD2 drug Solutions (Beijing, China).Equipments and Chromatographic ConditionsThe examination was carried out on the Waters 2695 Alliance HPLC technique (Waters Corp., Milford, MA, USA), outfitted having a quaternary solvent delivery technique, a web-based degasser, an autosampler, a thermostated compartment and also a 2996 photodiode array detection. Information assortment and integration have been accomplished by EmpowerTM Application (Waters Corp., Milford, MA, USA). All analytes had been separated on a LunaC18 (two) column (250.six mm, 5.0 , Phenomenex) and also a C18 guard column was applied before the analytical column. The separation was carried out with gradient elution procedure and mobile phase A (acetonitrile) and B ( phosphoric acid water alternative, adjusted with triethylamine to pH five.6) ratios linear transformed as follows: 0 15 min, 23 A; 15 26 min, 23 40 A; 26 65 min, 40 65 A. The complete run time was 65 min at a movement fee of one mLmin. The next HPLC parametersTable 1: A summary with the examined YZP samplesSample no. T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 C1 C2 S1 S2 S3 D1 D2 Business manufacturer Tongrentang, Tianjin Tongrentang, Tianjin Tongrentang, Tianjin Longzhong, Hubei Longzhong, Hubei Banzhou Ttianlong, Guangxi Duanxintang Dayu, Shandong Duanxintang Dayu, Shandong Hebang Yangguang, Sichuan Hebang Yangguang, Sichuan Rongyu, Jiangsu Rongyu, Jiangsu Kangyuan, Jiangsu Kangyuan, Jiangsu Kangyuan, Jiangsu Longshen Rongfa, Gansu Longshen Rongfa, Gansu Production date 2012-04-11 2012-06-28 2013-03-08 2013-01-03 2013-03-03 2012-11-02 2012-01-18 2012-03-24 2012-12-24 2012-11-05 2012-06-12 2012-08-04 2012-03-09 2012-10-05 2011-12-15 2012-05-20 2012-04-MATERIALS AND METHODSChemicals and ReagentsHPLCgrade acetonitrile and methanol had been obtained from Honeywell (Muskegon, MI, USA). HPLCgradeYZP: Yuanhu Zhitong prescriptionPharmacognosy Magazine | January-March 2015 | Vol eleven | IssueZhang, et al.: Quantitative Determination of Energetic Components in Yuanhu Zhitong Prescriptionab Figure 1: Chemical structures and chromatograms of 12 parts (protopine, PTE; -allocryptopine, ATP; coptisine, CTE; xanthotol, XTL; palmatine, PME; FGFR2 web dehydrocorydaline, DCE; glaucine, GCE; tetrahydropalmatine, THP; tetrahydroberberine, TDE; imperatorin, IMP; corydaline, CDE; isoimperatorin, ISO) (a); representative chromatograms and ultraviolet spectrogram for determination of 12 elements in four dosage types of YZP (b).have been applied for your evaluation: Column temperature, thirty ; injection volume, 20 , as well as the detection wavelength, 280 nm.Common stock solutionsRESULTS AND DISCUSSIONA Hypersil BDS C18 column (250 4.6 mm, 5.0 , elite) along with a Phenomenex LunaC18 (2) column (250 4.six mm, five.0 , Phenomenex) have been examined to optimize the chromatographic affliction. The resolution of Phenomenex LunaC18 (two) column was proved better than that of Hypersil BDS C18 column. Because of the main components of YZP are alkaloids, diverse mobile phases with buffer have been experimented with, including methanol ater (0.1 phosphoric acid water solution, adjusted with triethylamine to pH four, pH 4.5, pH 5, pH 5.5, pH 6), and acetonitrile ater ( phosphoric acid water option, adjusted with t.