Rmula, composed of Corydalis yanhusuo (Y.H.Chou and Chun C.Hsu) W.T.Wang ex Z.Y.Su and C.Y.Wu and Angelica dahurica (Hoffm.) Benth. and Hook.f. ex Franch. and Sav have been officially recorded in Chinese Pharmacopoeia for the treatment of stomach pain, hypochondriac pain, headache and dysmenorrhea brought on by qistagnancy and blood stasis.[6] Up to now,Zhang, et al.: Quantitative Determination of Active Components in Yuanhu Zhitong PrescriptionYZP RORĪ³ Agonist Storage & Stability happen to be extensively applied and made into numerous dosage forms such as tablets, capsules, soft capsules, oral liquids, granules, dropping tablets, etc., Tetrahydropalmatine is the only marker element for the good quality control of all dosage forms of YZP in Chinese Pharmacopoeia.[6] On the other hand, accumulating documentary records have indicated that alkaloids in Corydalis yanhusuo (Y.H.Chou and Chun C.Hsu) W.T.Wang ex Z.Y.Su and C.Y.Wu and coumarins in Angelica dahurica (Hoffm.) Benth. and Hook.f. ex Franch. and Sav have been the active elements of YZP.[711] The content of tetrahydropalmatine has already not revealed the real high quality of YZP. At the present time, a handful of analytical methods (HPLC, TLC, capillary electrophoresis (CE)) mainly focusing on tetrahydropalmatine (THP), protopine (PTE), imperatorin (IMP) or isoimperatorin (ISO)[1219] happen to be reported as quality assessment for YZP in China. One study[20] making use of rapid resolution liquid chromatography coupled with a triple quadrupole electrospray tandem mass spectrometry (RRLCQQQ) method determined 17 elements of YZP tablets. However, this study might not appropriate for other dosage forms due to diverse impurities in various dosage forms. To date, the strategies for the simultaneous separation and quantitative determination of a number of active components in a single running for other dosage forms of YZP are nonetheless not readily available. Hence, a universal strategy for the quantitative determination of various active components in distinctive dosage forms of YZP is needed and handy for their high quality control. Within the present study, a straightforward, precise, and trustworthy analytical system for quantitative determination o f 1 two a c t ive c o m p o n e n t s c o n t a i n e d i n Y Z P like protopine (PTE), allocryptopine (ATP), coptisine (CTE), Nav1.8 Antagonist Molecular Weight xanthotol (XTL), palmatine (PME), dehydrocorydaline (DCE), glaucine (GCE), THP, tetrahydroberberine (TDE), IMP, corydaline (CDE), ISO (they were chose based on our prior investigation,[21,22] Figure 1) was firstly created and validated applying a HPLC coupled having a photodiode array (PDA) detection. The results have indicated that the validated HPLCPDA strategy is quite basic, appropriate and universal for the routine analysis of four main dosage types (tablets, capsules, soft capsules, dropping tablets) of YZP and their good quality manage.phosphoric acid and triethylamine had been obtained from Tianjin Kermel Chemical Reagent Co., Ltd. The deionized water was prepared from Millipore water purification system (Milford, MA, USA) and filtered with a 0.22 membrane. 4 dosage forms containing 17 batches of YZP coming from eight manufacturers had been obtained from industrial sources [Table 1]. PTE, CTE, XTL, PME, GCE, TDE (98 , purity) had been acquired from Shanghai Tauto Biotech Co., Ltd. ATP was obtained from Shenzhen Meihe Biotech Co., Ltd. CDE and DCE (98 , purity) had been bought from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). THP, IMP and ISO had been obtained from National Institute for the Control of Pharmaceutical and Biological Merchandise.