supplying the very best sustained viral response amongst other combinations of directacting antiviral agents (DAAAs) by achieving 99 sustained viral response decompensated cirrhotic individuals inside a 12 week regimen.2,16 Alternatively, applying molecular docking analyses,9 Epclusa has shown to exhibit higher efficiency against COVID-19.six,7 This was ascribed for the similarity amongst nonstructural protein 12 (nsp12) in each HCV and SARSCOV-2 RNA polymerases, which was located to possess high prospective for SARS-COV-2 viral infection.six,2021 The Authors. Published by American Chemical SocietyThe necessity to monitor such a life-saving drug emerges from the truth that it is actually extensively metabolized by liver cytochrome P450 enzymes; CYP2B6, CYP2C8, and CYP3A4.17,18 This tends to make its metabolism prone to alteration by other inhibiting therapeutic agents that impact these enzymes. To this end, VLP determination within the presence of sofosbuvir was reported using a plethora of procedures, including LC-MS/MS,19,20 UPLC-ESCI MS/MS,21 HPLC-MS/MS,22 and HPTLC,23 in biological fluids and clinical studies. Also, the determination of the two drugs in their pharmaceutical formula was addressed utilizing UPLC,24 spectrofluorimetry,25 UPLC/spectrofluorimetry,26 and spectrophotometry.27 Having said that, the determination variety is somewhat high priced and time-consuming. Within this regard, electrochemistry can offer extremely sensitive and fast analysis tactics that are basic and cost-effective.28-33 The electrochemical determination of VLP applying the molecularReceived: August 19, 2021 Accepted: September 17, 2021 Published: September 30, ACS Omega 2021, 6, 26791-ACS Omega site acsodfArticleFigure 1. Schematic of your preparation of 5-BSA=N-MIL-53(Al) plus the recommended oxidation mechanism of VLP.Figure 2. FESEM images of (a) NH2-MIL-53 (Al) and (b) 5-BSA=N-MIL-53(Al), (c) XRD of each NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al), and (d) BET adsorption-desorption isotherms of NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al).imprinted polymer deposited on a 3D starfish-like hollow nickel skeleton decorated with the MWCNT-Au NP-modified glassy carbon electrode has lately been reported in pharmaceutical formula and biological fluids.34 Regardless of the achieved fantastic linearity (5.66-90.60 nM) and low limit of detection (LOD) (0.227 nM), the high price in the composite along with the tedious synthesis method render the entire procedure difficult to adopt for routine analysis.34 These limitations KDM2 list necessitate the search for option platforms. Herein, we demonstrate the usage of 5-bromo-salicylaldehydeNH2-MIL-53(Al) (5-BSA) as an effective and novel metal- organic framework (MOF) material with superior electrochemical properties. Note that NH2-MIL-53(Al) was shown tobe efficient in the detection of mercury ions in water with a low detection limit.35,36 Our modification of NH2-MIL-53(Al) yielded a much more extremely responsive MOF with superior conductivity. Especially, the fabricated 5-BSA=N-MIL-53(Al) was investigated as a sensor material for the electrochemical determination of VLP, recognizing that sofosbuvir is electrochemically inactive,37,38 in buffer solutions and biological fluids. Our proposed platform presents a low cost, feasible, and convenient system of preparing a sensor with the wide range of linearity (0.11-25.97 M), whilst providing a comparable LOD of eight.776 ACS Omega 2021, 6, 26791-ACS Omega