E values (number of individuals n= 39) obtained for 14 of 20 the Raman biomarker of cytochrome c I1584 we obtained a plot as a function of cancer grade malignancy.Figure 7. The Raman intensities of cytochrome c cytochrome b in b in human breast tissue = 39: I 39: I1584 I750 I750 I1126 Figure 7. The Raman intensities of cytochrome c and and cytochrome human breast tissue for n for n =1584 (A), (A), (B), (B), I1126 (C), I1337 (D) asfunction of of breast cancer grade malignancy G0-G3 excitation 532 nm (A). The results areare presented as (C), I1337 (D) as a a function breast cancer grade malignancy G0-G3 at at excitation 532 nm (A). The results presented as thethe meanSD. Raman bands intensity had been taken from normalized byby vector norm spectra. imply SD. Raman bands intensity were taken from normalized vector norm spectra.In the provides results presented in Figure 7 it response, generally the biomarker The plotview of thean significant cell-physiologic is evident that the Ramanreduced I1584 measuring contribution of cytochrome c in cells, nevertheless it breast tissues correlates with cytochrome c operates at low, basal level in typical the human is strongly p38 MAPK medchemexpress induced to pretty breast cancer aggressiveness. The intensity in the 1584 cm-1 Raman signal corresponding higher levels in pathological cancer states. to It’s level of reduced cytochrome c characteristic bands of cytochrome c and cythe interesting to check when the other increases with growing cancer aggressiveness. It indicates that cytochrome c plays important function inside the improvement and progression of tochrome b correlate with breast canceragrade malignancy G0-G3. cancer. The parabolicthe Raman signals of other characteristic vibrations of cytochromes Figure 7B,C shows dependence with the Raman biomarker I1584 of the decreased cytochrome c in as a function of breast cancer grade malignancy G0-G3 for the human tissues. 1 c and bFigure 7 vs cancer malignancy shows that the optimal concentration of cytochrome c can seeare necessary to retain cellular homeostasis corresponds tob (750 and 1126 intensity that that the bands of mixed vibrations of cytochromes c plus the normalized cm-1 ) increases with aggressiveness. The peaks at concentrations in the reduced cytochrome b at of 0.006 0.003 for the breast tissues. The 1337 cm-1 corresponding to cytochromes c doesn’t virtually modify with breast cancer aggressiveness. resulting in optimistic effects this level modulate protective, signaling-response pathways, onIt was reported [20,391] thatcytochrome c nm laser excitation the 0.06 0.02 triggers life-history traits. The reduced under 530.9 level above the value of Raman peak at 750 cm-1 runaway approach and aggressive breast cancer improvement. at 1126 cm-1 by a toxic was primarily determined by c-type cytochromes, TLR3 drug whereas peak b-type cytochromes. The plot gives a crucial cell-physiologic response, usually the reduced cyWe analyzed the at low, basal level in from the cells, nevertheless it cm-1 and induced -1 really tochrome c operatesRaman intensity ratio normalpeaks at 750is strongly 1126 cm to to evaluated the relative contribution ofstates. high levels in pathological cancer cytochrome c and b (see Figure 7B,C). Figure 8 shows the Raman intensity ratio on the peaks at 750 cm-1 and 1126 cm-1 in human tissue as a function of breast cancer grade malignancy G0-G3 at excitation 532 nm. A single can see from Figure 8 that the relative intensity ratio I750 /I1126 increases with breast cancer aggressiveness. It indicates that the.