Ing and immune-modulation functions to tackle tumour cells by numerous fronts although enabling imaging follow-up. Conclusions: Altogether, EVs might potentiate natural or induced immune response suggesting their use as nanocarriers in combinatorial therapeutic approaches. Likewise, cell membrane-derived gold nanoparticle-loaded nanoghosts show promising properties for their exploitation in cancer multivalent therapy.Scientific System ISEVPoster Session S01 EVs and Stem Cells II Chairs: TBD and Yaxuan LiangPS01.PPAR carried by microparticles restores the failed differentiation and functionality of bone marrow-derived cells induced by high-fat diet program Luisa Vergori1, Emilie Lauret2, Raffaella Soleti2, Ramaroson Andriantsitohaina1 and M. Carmen Martinez5:15:30 p.m.INSERM U1063; 2INSERM UMR1063 University of Angers, FranceMetabolic pathologies such as diabetes and obesity are connected with decreased amount of Basal Cell Adhesion Molecule (BCAM) Proteins Gene ID circulating and bone Endothelial Cell-Selective Adhesion Molecule (ESAM) Proteins site marrow (BM)-derived endothelial progenitor cells (EPCs). It is identified that activation of peroxisome proliferator-activated receptor (PPAR) may stimulate cell differentiation. Moreover, microparticles (MPs), smaller membranes vesicles created by activated and apoptotic cells, are able to reprogram EPCs. Here, we evaluated the part of PPAR carried by MPs on both phenotype and function of progenitor cells from mice fed with a high-fat diet regime (HFD). Male (C57BL/6N, 8 weeks-old) mice received either a normal or possibly a high-fat diet program (HFD) (42 kcal from fat) for 12 weeks. Bone marrow (BM)-derived cells have been obtained from femurs and tibias of mice and cultured inside the absence or in the presence of MPs taken either from wild-type (PPAR+/+) or PPAR knock out (PPAR-/-) mice for 7 days. Characterisation of cells was performed by flow cytometry. The effects of MPs in vivo neovascularisation had been studied by Matrigel plug assay. We observed that HFD induced hyperglycemia and dyslipidemia, and decreased circulating EPCs. Immediately after 7 days of culture, BM-derived EPCs and monocytic progenitor cells from HFD-fed mice displayed impaired differentiation. In the similar time, we show that MPs bearing PPAR, MPsPPAR+/+, elevated the differentiation of EPCs and monocytic progenitors from HFD-fed mice, whereas MPsPPAR-/- had not impact on the differentiation of all varieties of progenitor cells. Additionally, MPsPPAR+/+ improved the capacity of progenitor cells to promote in vivo angiogenesis in mice fed with HFD. The in vitro and in vivo effects of MPsPPAR+/+ were abolished in presence of PPAR inhibitor, MK886. These data highlight the capability of PPAR carried by MPs to restore the failed differentiation and functionality of BM-derived cells induced by HFD.sensitive and -resistant GSC lines, and analysed by nanoparticle tracking analysis (NTA) and mRNA expression profiles. Results: Person tumours derived from the very same isogenic GSC line expressed divergent profiles of TMZ resistance markers, using a minor representation of your O6-methyl guanine DNA methyltransferase (MGMT). The changes in mRNA profiles, reflective of TMZ resistance and stemness expressed by chemo-resistant GSCs, were recapitulated within the transcriptome of exosome-like EVs released by these cells into the culture medium. In addition a important raise within the number of EVs released was observed in 2 more than 3 TMZ-resistant variants in comparison to TMZ-sensitive GSCs. Conclusion: Hence, GBM tumour initiating cells harbour many alternative programmes that translate into chemotherapy resistance in viv.