The angiogenic and therapeutic added benefits related with CD34+ stem cell therapy.Trafficking research using confocal imaging and flow cytometry analyses revealed that ENPP-3 Proteins Source CD34Exo was selectively internalised by endothelial cells and cardiomyocytes relative to fibroblasts in the CD34Exo-injected ischemic hearts. MicroRNA expression profiling and Taqman assays indicated that CD34Exo are significantly enriched with pro-angiogenic miRNAs which include miR126. CD34Exo injection induced the expression of miR126 and several pro-angiogenic mRNAs in mouse ischemic myocardium, Leukocyte Elastase Inhibitor Proteins Biological Activity suggesting a direct transfer of miR126. CD34Exo lacking in miR126 had decreased angiogenic and therapeutic activity each in vitro and in vivo indicating that miR126 was critical for CD34Exo function.OS20.Mesenchymal stem cells and their secreted exosomes exert therapeutic effects in Duchenne muscular dystrophy Ariel Bier1, Peter Bernstein1, Simona Cazacu2, Amir Dori3 and Chaya Brodie4 Bar-Ilan University, Israel; 2Henry Ford Health Systems, Detroit, MI, USA; Sheba Medical Centre, Israel; 4Faculty of Life Sciences Bar-Ilan University, Israel and Neurosurgery Division, Henry Ford Health Systems, Detroit, MI, USA3OS20.Angiogenic mechanisms of human CD34+ stem cell exosomes within the repair of ischemic heart Yaxuan Liang1, Prabhu Mathiyalagan1, Sol Misener2, Douglas Losordo3 and Susmita Sahoo1 Cardiovascular Research Center, Icahn College of Medicine at Mount Sinai, New York, USA; 2Feinberg Cardiovascular Investigation Institute, Feinberg School of Medicine, Northwestern University, NY, USA; 3Caladrius BiosciencesIntroduction: Locally transplanted human CD34+ stem cells have already been shown to improve exercising tolerance in individuals with myocardial ischemia and promote angiogenesis in animal models. In an earlier study, very first of its kind, we’ve demonstrated that CD34+ cells secrete exosomes (CD34Exo) that constitute a vital component on the pro-angiogenic paracrine activity of your cells. Here, we investigated the mechanisms of CD34Exo-mediated repair in the ischemic myocardium and therapeutic angiogenesis by studying their miRNA content and uptake.Duchenne muscular dystrophy (DMD) can be a progressive lethal, X-linked disease of skeletal and cardiac muscle tissues brought on by mutation on the dystrophin gene, which leads to muscle degeneration. Cell therapy utilizing diverse cell forms has been regarded a potential therapeutic approach for the treatment of DMD. Mesenchymal stromal cells (MSCs) are obtained from autologous bone marrow and adipose tissues or from allogeneic placenta and umbilical cord. The safety and therapeutic impact of MSCs have already been demonstrated in pre-clinical and clinical studies and are attributed to paracrine effects that are partly mediated by extracellular vesicles. Here, we studied the therapeutic effects of MSCs and their secreted exosomes using human in vitro illness models of skeletal muscle cultures derived from wholesome and Duchenne patients and MDX mice. Treatment of satellite cells with conditioned media or exosomes secreted by MSCs increased the proliferation and generation of PAX7+/MyoD+ cells as well as the differentiation of human myoblasts from both healthful and DMD individuals. MSCs from various sources exerted differential effects on the function in the muscle cells. Secretome and RNA sequencing analysis of the MSC-derived exosomes revealed particular cytokines and clusters of miRNAs and lengthy non-coding RNAs that were associated with anti-inflammatory and pro-regenerative activitie.