Ot study Jean-Luc Fraikin1; Marcy Maguire2; Franklin Monzon1; Richard ScottSpectradyne LLC, Torrance, USA; 2IVI-RMA International, Basking Ridge, USAPT02.Maternal serum extracellular RNA as noninvasive biomarkers related with abnormally invasive placenta Victoria Fratto1; Srimeenakshi Srinivasan1; Cuong To1; Peter De Hoff1; Vy Tran1; Allison O’Leary2; Melissa Westermann3; Mary Norton2; Deborah Wing3; Gladys E2 Enzymes Proteins medchemexpress Ramos1; Louise C. LaurentUniversity of California San Diego, San Diego, USA; 2University of California San Francisco, San Francisco, USA; 3University of California Irvine, Irvine, USABackground: Use of ultrasound and magnetic resonance imaging to diagnose abnormally invasive placenta (AIP) is pricey, imprecise and calls for specialized training. Extracellular RNAs (exRNAs) secreted below both physiological and pathological circumstances regulate gene expression post-transcriptionally. Recent research have focused around the possible use of exRNAs as biomarkers in numerous human diseasesBackground: Advances in in vitro fertilization have allowed top-rated fertility clinics to guarantee an about 70 likelihood of live birth from transfer of a single euploid embryo. Despite these great improvements, approximately one third of euploid embryos fail to implant. Exosomes have not too long ago been suggested to play roles in embryo implantation. Nevertheless, due to the fact embryos are grown in a low volume of complex media (usually 25 ), accurate quantification of exosomes in embryo culture has been challenging. In this early-stage pilot study, microfluidic resistive pulse sensing (MRPS) was utilised to predict embryo implantation by quantifying exosomes in the spent culture media of 20 human embryos. Approaches: Informed consent was obtained for use of supplies in this study. Spent media from blastocysts grown in single culture was collected and stored at -80 . Spent media from 10 embryos that RIO Kinase 1 Proteins web successfully implanted and 10 embryos that failed to implant were submitted for blinded analysis by MRPS. Samples had been thawed to room temperature and 3 taken from every single for evaluation. Total nanoparticle concentration was measured over the size variety 250000 nm diameter and was made use of to predict pregnancy outcome making use of a threshold established in the data. As a preliminary assessment of variability inside the MRPS measurements, one sample was measured in triplicate. Outcomes: MRPS analysis predicted pregnancy outcome with 80 sensitivity and 80 specificity. Particle concentration showed an approximate power-law dependence on size in each sample. Total nanoparticle concentration across samples clustered in two groups spanning approximately 1.2 E7 to 7.3 E7 particles/ml, with larger concentration in media from successfully implanted embryos. Preliminary assessment of variability in concentration measurements working with one particular sample showed CV three . Summary/conclusion: Within this pilot study, exosome concentrations in spent culture media measured by MRPS correlated strongly with embryo implantation potential a tantalizing result. On the other hand, additional in-depth validation is expected, and consistency of measurement outcomes will have to nonetheless be demonstrated extra broadly. If these metrics could be happy, MRPS could prove a important tool in predicting embryo implantation potential.Thursday, 03 MayPT02.Isolation and characterization of human seminal plasma exosomes: cars involved in spermatozoa motility properties and capacitation Valentina Murdica1; Greta Cermisoni2; Alessandro Bartolacci2; Elisa Giacomini2; Alessandr.