Alyzed various merchandise have been observed. According the mixture was analyzed by NMR; threedifferent goods have been observed. According toAccording to NMR spectra the crude reaction mixture, trisulfonated, disulfonated and NMR spectra obtained from the crudefrom the crude reaction mixture, trisulfonated, disulfonated NMR spectra obtained from obtained reaction mixture, trisulfonated, disulfonated and monosulfonatedmonosulfonated emodinHowever, the composition of your mixture changed monosulfonatedemodin were formed. Even so, theHowever, the of the mixture changed chan emodin were formed. were formed. composition composition in the mixture with time and reverse sulfonation occurred. Hence, Olesoxime custom synthesis work-up process, procedure, with time and reverse sulfonation occurred. Therefore, following thefollowing the work-upthe with time and reverse sulfonation occurred. Therefore, following the work-up process, crude reaction mixture in stirred in MeCN/hexane three the 3 h was was the crude reaction mixture was stirredMeCN/hexane and right after afterthehproduct the solution was co crude reaction mixture was stirred in was MeCN/hexane and three hand immediately after product completely a steady monosubstituted E_SO3 H, which was which as brown fully converted a converted to a stable monosubstituted E_SO3H,isolatedwasaisolated as a bro pletely converted to tostable monosubstituted E_SO3H, which was isolated as a brown strong in 76 strong in 76 yield (Figure three).yield (Figure three). solid in 76 yield (Figure three).Figure three. Synthesis of E_SO3 H. Figure 3. Synthesis of E_SO3H. Figure three. Synthesis of E_SO3H.1,3,8-Trimethoxy-6-methyl-9,10-anthraquinone obtained in obtained in 1,3,8-Trimethoxy-6-methyl-9,10-anthraquinone E_OCH3 was E_OCH3 wasgood yield great y 1,three,8-Trimethoxy-6-methyl-9,10-anthraquinone E_OCH3 was obtained (MeCO3 )yield (81 ) by applying a previously described reaction procedure in 2 2SO4 and base K2C (81 ) by applying a previously described reaction procedure (Me2 SO4 and base Kgood [30]. (81 ) by applying aBrominated trimethoxyemodin E_Br_OCH3 was 2synthesized from CO3 [30]. previously described reaction procedure (Me SO4 and base K2 tetrabromina Brominated trimethoxyemodin E_Br_OCH3 was synthesized from tetrabrominated emodin) emodin E_4Br by the E_Br_OCH3 Soon after purification, the item E_4Br_OCH [30]. Brominated trimethoxyemodinsame process. was synthesized from tetrabrominated 3 was tained in 87 yield. emodin E_4Br by the same system. Right after purification, the item E_4Br_OCH3 was obtained in 87 yield. two.2. Antiviral Activity of Emodin AnaloguesThe major objective two.two. Antiviral Activity of Emodin Analogues of this study was to figure out the possible of a library o emodin analogues (Figure 4) to combat the cytopathic Aztreonam custom synthesis effects of HCoV-NL63. The nMolecules 2021, 26,7 ofE_4Br by the same strategy. Immediately after purification, the solution E_4Br_OCH3 was obtained in 87 yield. two.two. Antiviral Activity of Emodin Analogues The major objective of this study was to identify the potential of a library of eight of 17 analogues (Figure 4) to combat the cytopathic effects of HCoV-NL63. The nontransformed primate kidney Vero cell line was utilised for this function. An added 8 of 17 series Molecules 2021, 26, x FOR PEER Critique of research was performed to evaluate the effects of every single compound around the viability of Vero cells.Molecules 2021, 26, x FOR PEER Critique 11 emodinFigure 4. Emodin and emodin derivatives prepared within the course of this study. Figure four. Emodin and emodin derivatives ready in the c.