Inal data. Empirical CDF is fitted to a lognormal LM22A-4 dose NS-018.html”>buy NS-018 distribution of mean ?and s.d. (dotted black line); fit is rejected at level 5 (p = 0.01). (b) Empirical CDF of f < 350 kPa (solid black line) with fitted normal distribution of ?and in kPa (dotted black line), not rejected at level 5 (p = 0.33). (c) Empirical CDFs of f for molecular motors (blue line, fitted lognormal not rejected) and non-molecular motors (red line, fitted lognormal rejected); the two distributions are not significantly different (p = 0.40). (d) Empirical CDFs (solid line) and fitted normal CDFs (dotted line) for molecular (blue line) and non-molecular (red line) motors with f < 350 kPa; ?and in kPa; the two distributions are not significantly different (p = 0.20). All comparisons based on Kolmogorov mirnov tests.2000 specific tension f in kPa0 RN DC DA KI MY SP PI FA FL MF FI MU MV motor typesFigure 2. Boxplots of specific tensions per motor type (n = 349). The boxes extend from the lower quartile to the upper quartile values with the medians (red line) in between. The whiskers extend to the most extreme data values within 1.5 ?IQR. Outliers (red crosses) are tensions beyond the end of the upper whiskers. Motor types: RN, RNA polymerase (n = 1); DC, cytoplasmic dynein (4); DA, axonemal dynein (4); KI, kinesin (7); MY, myosin (11); SP, spasmoneme (3); PI, pili (6); FA, F 0 /F 1 ATPase (2); FL, flagellum (4); MF, myofibril (16); FI, muscular fibre (97); MU, muscle in vitro (89); MV, muscle in vivo (105). ANOVA and multiple comparison of means (electronic supplementary material, table S1, motor types with n < 5 removed: RN, DC, DA, SP, FA and FL): PI = (KI, MY, MF, FI, MU, MV), FI = MV and MU = MV. Pili PI are significantly different from all other motor types.(a)1200 specific tension f (kPa) 1000 800 600 400 200 0 M1 M2 MF FI motor types MU MV(b)1200 1000 800 600 400 200 0 non-loc M1 M2 MF FI MU MV locomotor typesrsos.royalsocietypublishing.org R. Soc. open sci. 3:................................................(c)1200 specific tension f (kPa) 1000 800 600 400 200 0 Ba In Cr Mo Fi Am Re Bi Ma taxonomic groups(d )1200 1000 800 600 400 200 0 non-loc In Cr Fi Am Re Bi Ma taxonomic groups of locomotors(e)1200 specific tension f (kPa) 1000 800 600 400 200 0 non-loc Swim Terr motor functions FlyFigure 3. Boxplots of specific tensions of all motors except pili (n = 343). Pili were excluded from molecular assemblies (M2), bacteria (Ba) and terrestrial motors (Terr). (a) Per motor type. Abbreviations and number of values per class as defined in figure 2, except M1, single molecule (n = 27) and M2, molecular assembly (n = 9). ANOVA and multiple comparison of means (electronic supplementary material, table S2): MV = (M1, FI, MU). Among the 11 MV outliers, 9 are claw muscles and 2 are jump muscles. (b) Same as (a) with non-locomotors (non-loc, n = 55) as a separate class. ANOVA and multiple comparison of means (electronic supplementary material, table S3): non-loc = (M1, FI) and FI = MV. (c) Taxonomic groups: Ba, bacteria (n = 7); Pr, protozoa (4); Al, algae (1); Fu, fungi (1); Ec, echinoderms (1); Ar, arachnids (1); In, insects (19); Cr, crustaceans (19); Mo, molluscs (5); Fi, fish (29); Am, amphibian (31); Re, reptiles (5); Bi, birds (18); Ma, mammals (202). Groups with n < 5 (protozoa, algae, fungi, echinoderms, arachnids) were removed (remaining data: n = 335); ANOVA and multiple comparison of means (electronic supplementary material, table S4): crustaceans are significant.Inal data. Empirical CDF is fitted to a lognormal distribution of mean ?and s.d. (dotted black line); fit is rejected at level 5 (p = 0.01). (b) Empirical CDF of f < 350 kPa (solid black line) with fitted normal distribution of ?and in kPa (dotted black line), not rejected at level 5 (p = 0.33). (c) Empirical CDFs of f for molecular motors (blue line, fitted lognormal not rejected) and non-molecular motors (red line, fitted lognormal rejected); the two distributions are not significantly different (p = 0.40). (d) Empirical CDFs (solid line) and fitted normal CDFs (dotted line) for molecular (blue line) and non-molecular (red line) motors with f < 350 kPa; ?and in kPa; the two distributions are not significantly different (p = 0.20). All comparisons based on Kolmogorov mirnov tests.2000 specific tension f in kPa0 RN DC DA KI MY SP PI FA FL MF FI MU MV motor typesFigure 2. Boxplots of specific tensions per motor type (n = 349). The boxes extend from the lower quartile to the upper quartile values with the medians (red line) in between. The whiskers extend to the most extreme data values within 1.5 ?IQR. Outliers (red crosses) are tensions beyond the end of the upper whiskers. Motor types: RN, RNA polymerase (n = 1); DC, cytoplasmic dynein (4); DA, axonemal dynein (4); KI, kinesin (7); MY, myosin (11); SP, spasmoneme (3); PI, pili (6); FA, F 0 /F 1 ATPase (2); FL, flagellum (4); MF, myofibril (16); FI, muscular fibre (97); MU, muscle in vitro (89); MV, muscle in vivo (105). ANOVA and multiple comparison of means (electronic supplementary material, table S1, motor types with n < 5 removed: RN, DC, DA, SP, FA and FL): PI = (KI, MY, MF, FI, MU, MV), FI = MV and MU = MV. Pili PI are significantly different from all other motor types.(a)1200 specific tension f (kPa) 1000 800 600 400 200 0 M1 M2 MF FI motor types MU MV(b)1200 1000 800 600 400 200 0 non-loc M1 M2 MF FI MU MV locomotor typesrsos.royalsocietypublishing.org R. Soc. open sci. 3:................................................(c)1200 specific tension f (kPa) 1000 800 600 400 200 0 Ba In Cr Mo Fi Am Re Bi Ma taxonomic groups(d )1200 1000 800 600 400 200 0 non-loc In Cr Fi Am Re Bi Ma taxonomic groups of locomotors(e)1200 specific tension f (kPa) 1000 800 600 400 200 0 non-loc Swim Terr motor functions FlyFigure 3. Boxplots of specific tensions of all motors except pili (n = 343). Pili were excluded from molecular assemblies (M2), bacteria (Ba) and terrestrial motors (Terr). (a) Per motor type. Abbreviations and number of values per class as defined in figure 2, except M1, single molecule (n = 27) and M2, molecular assembly (n = 9). ANOVA and multiple comparison of means (electronic supplementary material, table S2): MV = (M1, FI, MU). Among the 11 MV outliers, 9 are claw muscles and 2 are jump muscles. (b) Same as (a) with non-locomotors (non-loc, n = 55) as a separate class. ANOVA and multiple comparison of means (electronic supplementary material, table S3): non-loc = (M1, FI) and FI = MV. (c) Taxonomic groups: Ba, bacteria (n = 7); Pr, protozoa (4); Al, algae (1); Fu, fungi (1); Ec, echinoderms (1); Ar, arachnids (1); In, insects (19); Cr, crustaceans (19); Mo, molluscs (5); Fi, fish (29); Am, amphibian (31); Re, reptiles (5); Bi, birds (18); Ma, mammals (202). Groups with n < 5 (protozoa, algae, fungi, echinoderms, arachnids) were removed (remaining data: n = 335); ANOVA and multiple comparison of means (electronic supplementary material, table S4): crustaceans are significant.