providing the best sustained viral response among other combinations of directacting antiviral agents (DAAAs) by achieving 99 sustained viral response decompensated BRPF2 custom synthesis cirrhotic individuals inside a 12 week regimen.2,16 On the other hand, making use of molecular docking analyses,9 Epclusa has shown to exhibit high efficiency against COVID-19.6,7 This was ascribed towards the similarity among nonstructural protein 12 (nsp12) in both HCV and SARSCOV-2 RNA polymerases, which was found to possess higher prospective for SARS-COV-2 viral infection.6,2021 The Authors. Published by American Chemical SocietyThe necessity to monitor such a life-saving drug emerges in the truth that it is extensively metabolized by liver cytochrome P450 enzymes; CYP2B6, CYP2C8, and CYP3A4.17,18 This tends to make its metabolism prone to alteration by other inhibiting therapeutic agents that affect these enzymes. To this finish, VLP determination within the presence of sofosbuvir was reported utilizing a plethora of methods, including LC-MS/MS,19,20 UPLC-ESCI MS/MS,21 HPLC-MS/MS,22 and HPTLC,23 in biological fluids and clinical studies. Additionally, the determination with the two drugs in their pharmaceutical formula was addressed utilizing UPLC,24 spectrofluorimetry,25 UPLC/spectrofluorimetry,26 and spectrophotometry.27 On the other hand, the determination variety is somewhat high priced and time-consuming. Within this regard, electrochemistry can deliver extremely sensitive and speedy evaluation procedures that are very simple and cost-effective.28-33 The electrochemical determination of VLP employing the molecularReceived: August 19, 2021 Accepted: September 17, 2021 Published: September 30, ACS Omega 2021, six, 26791-ACS Omega 1. Schematic from the preparation of 5-BSA=N-MIL-53(Al) along with the recommended oxidation mechanism of VLP.Figure two. FESEM pictures of (a) NH2-MIL-53 (Al) and (b) 5-BSA=N-MIL-53(Al), (c) XRD of both NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al), and (d) BET adsorption-desorption isotherms of NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al).imprinted polymer deposited on a 3D starfish-like hollow nickel skeleton decorated using the MWCNT-Au NP-modified glassy carbon electrode has lately been reported in pharmaceutical formula and biological fluids.34 Regardless of the ALK5 Accession accomplished great linearity (5.66-90.60 nM) and low limit of detection (LOD) (0.227 nM), the high expense of the composite and the tedious synthesis approach render the whole method hard to adopt for routine evaluation.34 These limitations necessitate the search for option platforms. Herein, we demonstrate the usage of 5-bromo-salicylaldehydeNH2-MIL-53(Al) (5-BSA) as an efficient and novel metal- organic framework (MOF) material with superior electrochemical properties. Note that NH2-MIL-53(Al) was shown tobe efficient inside the detection of mercury ions in water with a low detection limit.35,36 Our modification of NH2-MIL-53(Al) yielded a far more very responsive MOF with superior conductivity. Especially, the fabricated 5-BSA=N-MIL-53(Al) was investigated as a sensor material for the electrochemical determination of VLP, understanding that sofosbuvir is electrochemically inactive,37,38 in buffer options and biological fluids. Our proposed platform presents a inexpensive, feasible, and convenient strategy of preparing a sensor together with the wide variety of linearity (0.11-25.97 M), although providing a comparable LOD of 8.776 ACS Omega 2021, 6, 26791-ACS Omega