Centrifuged at 20,000 g for 90 min at 18 . The pellet of PMPs loaded with DOX (PMPDs) was resuspended in PAS. The sizes as well as the concentrations of PMPs and PMPDs were measured utilizing a nanoparticle tracking analysis (NTA). Data were analysed employing NTA software program. Transportation of DOX from PMPDs to breast cancer cell lines was observed by deconvolution microscopy. Outcomes: NTA final results revealed that the imply size of PMPDs (234.1 48.01 nm) was slightly bigger compared with that of PMPs (200.1 57.71 nm) and that DOX incorporation did not influence the quantification of PMPs. The concentration of them was no substantial difference. The size distributions and pictures of PMPs and PMPDs indicated the absence of aggregated PMPs connected with DOX loading. When incubated with MCF-7 and MDA-MB-231 cells, PMPDs transferred DOX to the nuclei of cancer cells within 30 min. Summary/Conclusion: These final results assistance the potential clinical use of PMPDs as novel cell-based “Trojan Horse” anti-cancer Adenosine A3 receptor (A3R) Inhibitor Formulation therapeutic method. Funding: This study was supported by the Ministry of Science and Technologies.PT11.Design and style of an exosome-based drug delivery program transporting anticancer peptides for targeting breast metastases in the brain Filipa Oliveiraa, Julia Skalskaa, Tiago Figueiraa, Patr ia Napole a, ica Mellob, David Andreuc, Valdirene Gomesb, Miguel Castanhoa and Diana Gaspara Instituto de Medicina Molecular Jo Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; bLaborat io de Fisiologia e Bioqu ica de Microrganismos do Centro de Bioci cias e Biotecnologia da Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, Brazil; 3Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Study Park, Barcelona, Spainacharacterized with transmission electron microscopy (TEM), atomic force microscopy (AFM), flow cytometry, Western Blot and dynamic light scattering. The interaction of PvD1 and vCPP2319 ACPs with the breast cells and respective exosomes was also followed with surface plasmon resonance (SPR) as to detail peptide’s binding to the different exosomes. Results: Outcomes suggests an intracellular target for vCPP2319 cytotoxic activity on breast cancer cells. The binding on the peptides to both membranes of human cells and exosomes results in cell death and in powerful binding, respectively, pointing to the potential ability of those breast exosomes in transporting ACPs, which in turn are highly productive towards tumour cells. Summary/Conclusion: Despite the fact that a lot more studies are at present in development, the combination of possible ACPs with human-derived exosomes are shown as a possible TRPA manufacturer source for a highly selective and effective DDS aiming to attack breast tumour cells situated inside the brain. Funding: Funda o para a Ci cia e a Tecnologia (FCT I.P., Portugal) is acknowledged for funding PTDC/BBBBQB/1693/2014. F. O., J. S. and T. F. acknowledge FCT I.P., Portugal for fellowships PD/ BD/135046/2017, PD/BD/114177/2016 and SFRH/BD/ 5283/2013, respectively. Marie Sklodowska-Curie Investigation and Innovation Employees Exchange (RISE) is acknowledged for funding: get in touch with H2020-MCA-RISE2014, Grant agreement 644, 167, 2015019.PT11.Embryonic stem cells-derived exosomes endowed with targeting properties as chemotherapeutics delivery autos for glioblastoma therapy Xiaozheng Ling, Qingwei Zhu, Yunlong Yang, Yang Wang, Zhifeng Deng Shanghai Jiao Tong University Affliated Sixth People’s Hospital, Shanghai, Chin.