Product Name: SPARCL1 / MAST9 Antibody, Mouse MAb
Immunogen: Recombinant Human SPARCL1 protein
Mouse IgG1: Mouse IgG1
Concentration:
Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 . The IgG fraction of the cell culture supernatant was purified by Protein A aff
Specificity: Human SPARCL1 / MAST9No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.Application
Application: ELISA, ICC/IF, IF, FCMELISA: 0.5-1 μg/mLThis antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SPARCL1. The detection limit for SPARCL1 is 0.0195 ng/well.FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mLStorage
CAS NO.: 1235034-55-5
Product: A-1155463
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (
Caption: Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (15 µg/ml). Then cells were stained
Background: SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular protein
PubMed ID:http://jpet.aspetjournals.org/content/201/2/357