Product Name: Anti-V5 Tag Antibody, Mouse Mab
Immunogen: A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
Mouse IgG1: Mouse IgG1
Concentration:
Formulation: 0.2 μm filtered solution in PBS
Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supe
Specificity: Recognize N-terminal and C-terminal V5 Tag in fusion proteinsApplication
Application: ICC/IF, IFICC/IF: 10-25 μg/mLStorage
CAS NO.: 115103-85-0
Product: MK-571 (sodium salt)
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (
Caption: Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% T
Background:
PubMed ID:http://jpet.aspetjournals.org/content/200/2/271